2X YourTaq™ Hot-Start PCR Master Mix, lyophilized

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Transportní podmínky                                

pokojová teplota (RT)                                     

Features

• Stable enzyme and mix for ambient shipment and room-temperature storage
• Exceptionally pure YourTaq Hot Start DNA Polymerase
• Optimized Master Mix for increased yield of amplification
• Resistant to PCR inhibitor carry-over
• Excellent PCR specificity and sensitivity for a broad range of amplicons

Applications

• Routine and demanding PCR amplification up to 3 kb
• Suitable for amplification of low target copy number
• TA cloning

biotechrabbit™ lyophilized YourTaq Hot Start PCR Mix is a freeze-dried version of the well-established liquid equivalent. The stabilized format allows shipment and storage without cooling. The Master Mix is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. YourTaq Hot Start PCR Mix shows excellent PCR specificity and sensitivity for a broad range of amplicons. The mix is resistant to PCR inhibitors, such as blood (up to 20%), Ethanol or humic acid enabling PCR amplification from DNA templates with carry-over of PCR-inhibitors.

The 2× YourTaq Hot Start PCR Mix contains pure biotechrabbit YourTaq Hot Start DNA Polymerase, extremely high-quality dNTPs and optimized PCR buffer; thus, only template, PCR primers and PCR-grade water are added. In addition, the mix is suitable for amplification of GC-rich templates (up to 70%) pairing with 5× PCR Enhancer (BR1900201).

Info: Recommended annealing temperature is 2°C above primer Tm (use gradient PCR to optimize the annealing temperature).

Catalog #	Size	Package Content
BR0202403	200 reactions of 50 µl	4 × 2X Lyo YourTaq Hot-Start PCR Master Mix 4 × 1.25 ml PCR Master Mix Reconstitution Buffer